The first release based on the Vocaloid 2 engine came from PowerFX with Sweet Ann on June 29, 2007 whose boxart was based on a Frankenstein's monster.This was closely followed by Crypton two months later with the first of their "Character Vocal Series", Hatsune Miku, on August 31, 2007. Zero-G's first Vocaloid, Prima, came out on January 14, 2008 with voice of a Soprano opera singer. Internet Co., Ltd. also joined the Vocaloid development with their first Vocaloid Gackpoid on July 31, 2008, whose voice was provided by Japanese international singer Gackt. Gackpoid includes a new program, OPUS Express, for mixing vocal parts with accompaniment or phoneme data.AH Software later released their first Vocaloids on December 4, 2009 bringing the total of studios producing Vocaloids to five. As of the introduction of Bplats and Sony Music Entertainment Japan in late 2010, there are seven studios involved with the production and distribution of Vocaloids with two involved solely in English, four solely in Japanese and one in both languages for which the software is developed.Crypton Future Media released the first dual Vocaloid Kagamine Len and Kagamine Rin, a Japanese male and female. On July 18, 2008, Crypton Future Media released the updated edition of Kagamine Rin and Len, named "act2".For a period of time, users who had bought the old version were allowed to get the new version for free. On June 18, 2008, beta demonstration songs using the new version were released on the company's official blog.The expansion disc is an entirely different software and does not affect the original Kagamine Rin/Len installation in any way, giving the user options to either use the old or new voice sets exclusively or combine their usage. Crypton Future Media have now retired the sale of their old Kagamine Vocaloid and it is now no longer possible to buy the software from them.This was also the very first Vocaloid update to be done for any Vocaloid.